It is the second high-level US group to visit while military tensions simmer between the self-ruled island and China.
Beijing, which claims democratically governed Taiwan as its own territory, has conducted military drills around the island to express its anger over US House of Representatives Speaker Nancy Pelosi's visit to Taipei in early August.
The defacto US embassy in Taipei said the delegation that arrived on Sunday is being led by Senator Ed Markey, who is accompanied by four House lawmakers on what it described as part of a larger visit to the Indo-Pacific region.
Taiwan's presidential office said the group would meet Tsai on Monday morning.
Senator Markey chairs the Senate Foreign Relations East Asia, Pacific, and International Cybersecurity Subcommittee.Â
Senator Markey's office said they "will reaffirm the United States' support for Taiwan ... and will encourage stability and peace across the Taiwan Strait."
The group will meet with elected leaders and members of the private sector to discuss shared interests including reducing tensions in the Taiwan Strait and expanding economic cooperation, including investments in semiconductors, the senator's office said.
While China's drills around Taiwan have abated, it is still carrying out military activities.
Eleven Chinese military aircraft crossed the Taiwan Strait's median line or entered Taiwan's air defence zone on Sunday, Taiwan's Defence Ministry said. Thirteen planes crossed the strait on Saturday, the ministry said.
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