A grand jury in Washington rejected prosecutors' request to charge Cassidy Hutchinson, according to two people familiar with the matter.
Details of prosecutors' allegations against Hutchinson were not clear, but Republican lawmakers had accused her of lying under oath in her explosive testimony in 2022 about Trump's behaviour on January 6, 2021, when a mob of his supporters stormed the Capitol to halt the congressional certification of his election loss to Democrat Joe Biden.
The effort to indict Hutchinson is part of a surge in actions by the Justice Department targeting perceived adversaries of the president, including a new attempt to prosecute former FBI director James Comey.
It's highly unusual for grand juries to decline to return indictments, but the Justice Department under Trump has been rebuked in a number of high-profile cases.
Hutchinson declined to comment on Tuesday.
Hutchison, who had been special assistant to Trump's chief of staff Mark Meadows, described an angry, defiant president who was trying to let armed protesters avoid security screenings at a rally to protest his 2020 election defeat and who later grabbed at the steering wheel of the presidential SUV when the Secret Service refused to let him go to the Capitol.
And when the events at the Capitol spiralled toward violence, she testified that Trump declined to intervene.
Trump at the time denied on his social media platform much of what Hutchinson said, calling her a "total phoney" and "bad news".
Republicans disputed her account, with House members releasing an interview with a Secret Service officer who drove the presidential SUV saying he did not see the altercation that Hutchinson described.
However, he did say the president had insisted on going to the Capitol.
Hutchinson alleged that members of Trump's inner circle dangled job opportunities and financial assistance while she was co-operating with the committee.
She also testified that a former ethics counsel in the Trump White House told her "the less you remember, the better."
The former lawyer disputed the account.
Trump pardoned nearly everyone charged with taking part in the Capitol attack on his first day back in office for a second term in January 2025.
Special counsel Jack Smith and his team secured an indictment in 2023, accusing Trump of plotting to overturn his defeat in the 2020 election.
Smith dropped that and another case after Trump won the 2024 election, citing a policy against prosecuting a sitting president.