A spokesperson for the Qatari Foreign Ministry told a press briefing that Iran should stop threats against Arab Gulf countries and open the Strait of Hormuz to show good intentions.
Qatar, along with Pakistan, has been mediating between the US and Iran in an effort to end the US-Israeli war on Iran that has disrupted global shipping and oil supplies.
Top officials from Saudi Arabia, Turkey and Pakistan also met Iranian officials in Tehran on Monday in an effort to de-escalate the conflict.
Tensions between the US and Iran have sharpened recently, with President Donald Trump saying on Friday the US may strike Iran again.
Iranian security officials told Reuters Iran had drawn up detailed plans to attack Gulf undersea cables if the US and Israel attack again.
Iranian Security Council chief Mohsen Rezaei on Tuesday met Supreme Leader Ayatollah Mojtaba Khamenei, not seen in public since succeeding his slain father in March, a day after holding talks with representatives of Saudi Arabia, Turkey and Pakistan.
State media said Khamenei had stressed the importance of pursuing Iran's interests and maintaining its conditions for ending the war.
Iran's state media said on Tuesday that "safeguarding national and regional security by maintaining control over the Strait of Hormuz and strengthening bilateral relations with neighbouring countries" were among the topics Khamenei and Rezaei had discussed.
Iran's conditions for a peace deal include an end to a US blockade of Iranian ports and US sanctions on Iranian oil exports.
US Vice President JD Vance said three weeks ago that Iran must reduce nuclear enrichment to end the war.
